卡尔帕因
酶谱
蛋白酵素
酪蛋白
生物化学
化学
基因亚型
钙蛋白酶抑制剂
染色
酶
分子生物学
生物
遗传学
基因
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 279-285
被引量:1
标识
DOI:10.1007/978-1-4939-7111-4_26
摘要
Casein zymography has become one of the gold standard assays for monitoring mammalian calcium-activated proteases (calpains) in purified enzyme, cell, or tissue samples. This calpain zymography method takes advantages of (1) casein is an excellent substrate for major isoforms of calpains (Calpain-1, 2 and 3), (2) the embedded casein is digested into small peptides where the calpain bands are located, thus creating a clear zone upon Commassie blue gel staining, and (3) the calpain isoforms have different gel mobility under native gel conditions. Casein zymography is also useful in studying reversibility of inhibitor binding to calpains.
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