High-sensitivity concentration detection of trace doxycycline in solution by molecular imprinting based on surface plasmon resonance technology

The abuse of doxycycline (DC) will lead to the presence of its residues in animals and water environments, which threatens human health. It is of great significance to detect the micro-content of DC in solution. Based on surface plasmon resonance (SPR) technology, we develop a detection method for trace DC in solution by modifying the SPR sensor chip with a molecular imprinting technique. The results show that the SPR sensor could specifically recognize DC in solution, and the detection limit of this method reaches 10 −6 µg/mL, which is 100 times lower than that of the ELISA method. The detection signal has good linearity in the concentration range of 10 −6 −10µg/mL. This method has the advantages of a low detection limit, large linear range, and high specificity for DC detection.