Exploration of the Mechanisms Underlying Yu's Enema Formula in Treating Ulcerative Colitis by Blocking the RhoA/ROCK Pathway based on Network Pharmacology, High-performance Liquid Chromatography Analysis, and Experimental Verification

溃疡性结肠炎 罗亚 阻塞(统计) 灌肠 药理学 化学 信号转导 生物化学 医学 内科学 计算机科学 疾病 计算机网络
作者
Binbin Liu,Jie Zhang,Xiaoqi Wang,Wei Ye,Jiaming Yao
出处
期刊:Current Pharmaceutical Design [Bentham Science Publishers]
卷期号:30 (14): 1085-1102 被引量:1
标识
DOI:10.2174/0113816128290586240315071044
摘要

Background: The traditional Chinese medicine formula, Yu's Enema Formula (YEF), has demonstrated potential in the treatment of Ulcerative Colitis (UC). Objective: This study aimed to unveil the anti-UC mechanisms of YEF. Methods: Utilizing public databases, we obtained YEF and UC-related targets. GO and KEGG analyses were conducted via clusterProfiler and Reactome. The STRING database facilitated the construction of the PPI network, and hub targets were selected using cytoHubba. We used R software for differential expression and correlation analyses, and molecular docking was performed with PyMOL and AutoDock. HPLC analysis identified the compounds in YEF. For in vivo validation, a UC rat model was employed. Results and Discussion: 495 YEF-UC overlapping targets were identified. GO and KEGG analyses indicated enrichment in exogenous stimuli response, peptide response, positive MAPK cascade regulation, interleukin- related signaling, and the TLR4 cascade. Hub targets included CTNNB1, JUN, MAPK1, MAPK3, SRC, STAT3, TLR4, TP53, and RELA, which were often interconnected. Molecular docking revealed quercetin's strong binding affinity with CTNNB1, MAPK1, MAPK3, SRC, STAT3, TLR4, and TP53, consistent with HPLC analysis. In vivo experiments suggested that YEF has the potential to alleviate UC symptoms and protect the intestinal mucosal barrier by inhibiting the RhoA/ROCK pathway. Conclusion: YEF may safeguard the intestinal mucosal barrier in UC by targeting CTNNB1, MAPK1, MAPK3, SRC, STAT3, TLR4, and TP53, while blocking the RhoA/ROCK pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
量子星尘发布了新的文献求助10
1秒前
1秒前
十六发布了新的文献求助10
1秒前
L112233发布了新的文献求助10
2秒前
2秒前
2秒前
2秒前
科研人完成签到,获得积分10
3秒前
贪玩的谷芹完成签到 ,获得积分10
3秒前
4秒前
JamesPei应助内向万天采纳,获得10
4秒前
深情新之完成签到,获得积分20
4秒前
shanekhost完成签到 ,获得积分10
4秒前
活力的彩虹完成签到 ,获得积分10
4秒前
5秒前
打打应助JY采纳,获得10
5秒前
彭于彦祖应助之乡坞采纳,获得500
5秒前
CipherSage应助Wei采纳,获得10
6秒前
蜡笔小新完成签到,获得积分10
6秒前
东方耀完成签到,获得积分10
6秒前
Yummy完成签到,获得积分10
6秒前
6秒前
6秒前
6秒前
王慧完成签到,获得积分10
6秒前
7秒前
7秒前
smottom应助科研蚂蚁采纳,获得10
7秒前
7秒前
背后白梦完成签到,获得积分10
8秒前
羊羊羊完成签到,获得积分10
8秒前
黎芜发布了新的文献求助20
9秒前
9秒前
9秒前
周周完成签到,获得积分10
9秒前
默11完成签到 ,获得积分10
10秒前
10秒前
10秒前
10秒前
10秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969383
求助须知:如何正确求助?哪些是违规求助? 3514211
关于积分的说明 11172730
捐赠科研通 3249476
什么是DOI,文献DOI怎么找? 1794909
邀请新用户注册赠送积分活动 875441
科研通“疑难数据库(出版商)”最低求助积分说明 804827