Synovial fluid-induced signaling alters ribosome activity pathways in two knee OA synovial fluid sub-groups

Purpose (the aim of the study): Characterization of osteoarthritis (OA) patient-specific molecular processes is essential for OA endotyping. Several studies stratified OA in a number of molecular endotypes, based on transcriptomic profiles of joint tissues. While joint tissue sampling provides insight into the underlying disease mechanisms, clinical implementation of such an approach is challenging due to invasiveness and potential damage to joint tissues. Alternatively, synovial fluid (SF) aspiration is considered a less invasive approach and more suitable for diagnostic purposes. Considering the compositional complexity of synovial fluid, we aimed to stratify OA based on SF-induced differential cellular signaling patterns.