Shifts in bacterial diversity characteristics during the primary and secondary fermentation stages of bio-compost inoculated with effective microorganisms agent

Microbial inoculation was an effective way to improve product quality of composting and solve traditional composting shortage. However, the effect mechanism of microbial inoculation on compost microorganisms remains unclear. Here, Shifts in bacterial community, metabolic function and co-occurrence network during the primary and secondary fermentation stages of bio-compost inoculated with effective microorganisms (EM) agent were analyzed by high-throughput sequencing and network analysis. Microbial inoculation promoted organic carbon transformation in early stage of secondary fermentation (days 27 to 31). The beneficial biocontrol bacteria were main dominant genera at the second fermentation stage. Microbial inoculation can be good for the survival of beneficial bacteria. Inoculation with microbes promoted amino acid, carbohydrate and lipid metabolism, and inhibited energy metabolism and citrate cycle (TCA cycle). Microbial inoculation could enhance complexity of bacterial network and enhance mutual cooperation among bacteria during composting.