卵母细胞
体外成熟
化学
细胞生物学
男科
卵母细胞激活
胚泡
下调和上调
胚胎
卵泡液
细胞凋亡
PI3K/AKT/mTOR通路
胚胎发生
信号转导
内科学
生物
内分泌学
基因
生物化学
医学
作者
Mingju Sun,Xiangyu Wang,F. Christopher Bi,Hongxiao Xiang,Ning Wang,Wenju Gao,Yixuan Liu,Zizhen Lv,Youwei Li,Yanjun Huan
标识
DOI:10.1016/j.ijbiomac.2024.130590
摘要
Oocyte in vitro maturation (IVM) based on the follicular fluid (FF) environment can exploit untapped resources, however, what FF factors regulate oocyte maturation remains unclear. This work demonstrated that serum and FF significantly promoted oocyte polar body extrusion (PBE) and subsequent embryo development, and FF was especially effective. Fibronectin 1 (FN1) was predicted as one potential candidate to regulate oocyte maturation by proteomics. FN1 transcription obviously decreased, and the protein expression significantly increased and migrated to plasma membrane or even outside during oocyte IVM. Treatment with 10 ng/mL FN1 significantly improved oocyte PBE rate. FN1 significantly upregulated the percentage of regular spindle morphology, downregulated the γ-H2AX level, decreased the levels of ROS and apoptosis, and increased GSH and mitochondrion contents by ameliorating the expression of corresponding genes. Moreover, FN1 significantly increased the p-PI3K level to enhance the activation of PI3K signaling pathway. In conclusion, this study discovers and confirms that FN1 is one factor in FF that significantly enhances oocyte maturation, and the underlying mechanism is that FN1 ameliorates oocyte nuclear and cytoplasmic maturation by promoting the activation of PI3K signaling pathway.
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