A selective fluorescence chemosensor: Thiosemicarbazide Schiff base derivative for detection of Ag+ ions in living cells

Ag+ ions can be colorimetric and fluorometrically detected in the aqueous acetonitrile medium with great selectivity using TOV. Observable to the naked eye, the synthesized compound exhibited an amazing fluorometric reaction in the presence of Ag+, changing from colorless to yellow. We examine the suggested Ag+ sensor's analytical performance characteristics with a considerable detection limit of 6.138×10−9 M. The chemosensor can be used for Ag+ measurement. According to the experiment results, TOV's fluorometric response to Ag+ is pH-dependent throughout a broad pH range of 1.0 to 10.0. Furthermore, EDTA can be used to repeatedly reverse the Fluorometric reaction of TOV + Ag+. Job's plot, HRMS, and 1H NMR titration experiments have all been used to study the mechanism of interaction between the TOV and Ag+ ions. These studies have validated the 1:2 binding stoichiometry between TOV and Ag+. Test paper-based detection, Measuring Ag+ in water samples, were three examples of the real-time use of Ag+ detection and chemosensor was used in MCF7 cells to assess cytotoxicity.