黄曲霉毒素
检出限
免疫分析
单克隆抗体
多克隆抗体
生物标志物
抗体
色谱法
真菌毒素
化学
食品科学
生物
免疫学
生物化学
作者
Jiayun Fu,Min Gu,Xiaoqian Tang,Zhang Qi,Peiwu Li
出处
期刊:Food Control
[Elsevier]
日期:2024-06-01
卷期号:160: 110317-110317
标识
DOI:10.1016/j.foodcont.2024.110317
摘要
The pre-aflatoxin protein biomarker PAB-01 is a key target for early predicting of aflatoxin occurrence risk. Improving its detection sensitivity is significant for the early evaluation of potential risks. In this study, we obtained the monoclonal antibody 6A6 against PAB-01 through hybridoma technology. A sandwich ELISA was developed using pairwise combinations of PAB-01 nanobody, monoclonal antibody, and polyclonal antibody. It was found that the nanobody as the capture antibody and monoclonal antibody as the detection antibody exhibited the highest sensitivity, with a detection limit of 0.92 ng/mL. Based on this, a rolling circle amplification-based immunoassay (RCA-EIA) was established, with a linear range of 0.056–41.152 ng/mL and a detection limit of 0.033 ng/mL. Recovery rates in spiked peanut and corn samples ranged from 94.49% to 127.50%. This method successfully identified the presence of the PAB-01 protein in maize samples 10 days prior to the aflatoxin production. Based on PAB-01 concentration classifications, the maize samples were categorized into different risk levels. This development is significant for early warning and risk stratification management of aflatoxin occurrence, providing a highly effective tool in ensuring food safety.
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