普鲁士蓝
双功能
免疫分析
纳米技术
拉曼光谱
化学
检出限
色谱法
材料科学
医学
抗体
生物化学
光学
物理
物理化学
催化作用
免疫学
电化学
电极
作者
Xianlong Zhang,Yiheng Shi,Di Wu,Lihua Fan,Jianghua Liu,Yongning Wu,Guoliang Li
出处
期刊:Food Chemistry
[Elsevier]
日期:2024-02-01
卷期号:434: 137455-137455
被引量:8
标识
DOI:10.1016/j.foodchem.2023.137455
摘要
Food allergy has posed a great threat for public health due to its rising prevalence worldwide, and thus sensitive and reliable food allergen monitoring methods is of great significance. In this study, we prepared a bifunctional core-shell gold@Prussian blue nanoparticles (Au@PBNP) nanozyme, which not only could serve as an alternative to natural peroxidase for colorimetric immunoassay, but also act as a unique Raman label in Raman-silent region (1800-2800 cm-1) for SERS analysis. By combining microfluidic device, smartphone, and portable Raman spectrometer, a new smartphone/SERS dual-readout microfluidic immunoassay platform was established for portable detection of food allergic protein (i.e., alpha-lactalbumin (α-LA)). The established method for detection of α-LA showed a LOD of 0.011 ng/mL in a liner range of 0.2-600 ng/mL. Furthermore, this method was also challenged in spiked food samples with good average recoveries, showing a great potential in practical applications.
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