Improving DNA mixtures analysis using compound markers composed of InDels and SNPs screened from the whole genome with next‐generation sequencing

索引 单核苷酸多态性 遗传学 DNA测序 生物 DNA 计算生物学 基因 基因型
作者
Mengyu Tan,Jiaming Xue,Qiushuo Wu,Yazi Zheng,Guihong Liu,Ranran Zhang,Mengna Wu,Jinlong Song,Yuanyuan Xiao,Dezhi Chen,Meili Lv,Miao Liao,Shengqiu Qu,Weibo Liang
出处
期刊:Electrophoresis [Wiley]
卷期号:45 (5-6): 463-473
标识
DOI:10.1002/elps.202300195
摘要

Abstract Next‐generation sequencing (NGS) allows for better identification of insertion and deletion polymorphisms (InDels) and their combination with adjacent single nucleotide polymorphisms (SNPs) to form compound markers. These markers can improve the polymorphism of microhaplotypes (MHs) within the same length range, and thus, boost the efficiency of DNA mixture analysis. In this study, we screened InDels and SNPs across the whole genome and selected highly polymorphic markers composed of InDels and/or SNPs within 300 bp. Further, we successfully developed and evaluated an NGS‐based panel comprising 55 loci, of which 24 were composed of both SNPs and InDels. Analysis of 124 unrelated Southern Han Chinese revealed an average effective number of alleles (A e ) of 7.52 for this panel. The cumulative power of discrimination and cumulative probability of exclusion values of the 55 loci were 1–2.37 × 10 –73 and 1–1.19 × 10 –28 , respectively. Additionally, this panel exhibited high allele detection rates of over 97% in each of the 21 artificial mixtures involving from two to six contributors at different mixing ratios. We used EuroForMix to calculate the likelihood ratio (LR) and evaluate the evidence strength provided by this panel, and it could assess evidence strength with LR, distinguishing real and noncontributors. In conclusion, our panel holds great potential for detecting and analyzing DNA mixtures in forensic applications, with the capability to enhance routine mixture analysis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
明理青丝完成签到,获得积分10
2秒前
香蕉觅云应助吃肯德基采纳,获得10
2秒前
7777发布了新的文献求助10
3秒前
4秒前
量子星尘发布了新的文献求助10
6秒前
9秒前
9秒前
10秒前
12秒前
13秒前
14秒前
深情安青应助科研通管家采纳,获得10
14秒前
及禾应助科研通管家采纳,获得10
15秒前
及禾应助科研通管家采纳,获得10
15秒前
赘婿应助科研通管家采纳,获得10
15秒前
彭于晏应助科研通管家采纳,获得10
15秒前
CipherSage应助科研通管家采纳,获得10
15秒前
orixero应助科研通管家采纳,获得10
15秒前
15秒前
15秒前
夏老师发布了新的文献求助10
15秒前
Jaychai完成签到,获得积分10
18秒前
犇骉发布了新的文献求助10
18秒前
满意凡桃发布了新的文献求助10
18秒前
伶俐的悒完成签到,获得积分10
19秒前
夏老师完成签到,获得积分10
21秒前
小曲完成签到 ,获得积分10
23秒前
安笙凉城完成签到,获得积分10
25秒前
25秒前
顾矜应助满意凡桃采纳,获得10
25秒前
独特秋凌发布了新的文献求助10
25秒前
2418478262关注了科研通微信公众号
26秒前
27秒前
pcr163应助标致的冷梅采纳,获得50
27秒前
stark完成签到,获得积分10
28秒前
脑洞疼应助安笙凉城采纳,获得10
29秒前
ss完成签到,获得积分20
30秒前
fsf发布了新的文献求助10
31秒前
ʚᵗᑋᵃᐢᵏ ᵞᵒᵘɞ完成签到,获得积分10
33秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
The Moiseyev Dance Company Tours America: "Wholesome" Comfort during a Cold War 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3973968
求助须知:如何正确求助?哪些是违规求助? 3518173
关于积分的说明 11193215
捐赠科研通 3254336
什么是DOI,文献DOI怎么找? 1797103
邀请新用户注册赠送积分活动 876812
科研通“疑难数据库(出版商)”最低求助积分说明 806023