Improving DNA mixtures analysis using compound markers composed of InDels and SNPs screened from the whole genome with next‐generation sequencing

索引 单核苷酸多态性 遗传学 DNA测序 生物 DNA 计算生物学 基因 基因型
作者
Mengyu Tan,Jiaming Xue,Qiushuo Wu,Yazi Zheng,Guihong Liu,Ranran Zhang,Mengna Wu,Jinlong Song,Yuanyuan Xiao,Dezhi Chen,Meili Lv,Miao Liao,Shengqiu Qu,Weibo Liang
出处
期刊:Electrophoresis [Wiley]
卷期号:45 (5-6): 463-473
标识
DOI:10.1002/elps.202300195
摘要

Abstract Next‐generation sequencing (NGS) allows for better identification of insertion and deletion polymorphisms (InDels) and their combination with adjacent single nucleotide polymorphisms (SNPs) to form compound markers. These markers can improve the polymorphism of microhaplotypes (MHs) within the same length range, and thus, boost the efficiency of DNA mixture analysis. In this study, we screened InDels and SNPs across the whole genome and selected highly polymorphic markers composed of InDels and/or SNPs within 300 bp. Further, we successfully developed and evaluated an NGS‐based panel comprising 55 loci, of which 24 were composed of both SNPs and InDels. Analysis of 124 unrelated Southern Han Chinese revealed an average effective number of alleles (A e ) of 7.52 for this panel. The cumulative power of discrimination and cumulative probability of exclusion values of the 55 loci were 1–2.37 × 10 –73 and 1–1.19 × 10 –28 , respectively. Additionally, this panel exhibited high allele detection rates of over 97% in each of the 21 artificial mixtures involving from two to six contributors at different mixing ratios. We used EuroForMix to calculate the likelihood ratio (LR) and evaluate the evidence strength provided by this panel, and it could assess evidence strength with LR, distinguishing real and noncontributors. In conclusion, our panel holds great potential for detecting and analyzing DNA mixtures in forensic applications, with the capability to enhance routine mixture analysis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Cheung2121发布了新的文献求助30
刚刚
1秒前
3秒前
谜记完成签到,获得积分10
3秒前
共享精神应助Cheung2121采纳,获得30
3秒前
光撒盐完成签到,获得积分10
4秒前
cowboy007完成签到,获得积分10
4秒前
张振宇完成签到 ,获得积分10
5秒前
zz发布了新的文献求助10
6秒前
zzq778发布了新的文献求助10
8秒前
黄怡婷完成签到 ,获得积分10
8秒前
Daisy应助科研通管家采纳,获得10
9秒前
机智苗应助科研通管家采纳,获得10
9秒前
香蕉觅云应助科研通管家采纳,获得10
9秒前
FashionBoy应助科研通管家采纳,获得10
9秒前
赘婿应助科研通管家采纳,获得10
9秒前
大模型应助科研通管家采纳,获得10
9秒前
yanmu2010应助科研通管家采纳,获得10
9秒前
kingwill应助科研通管家采纳,获得20
10秒前
银包铜应助科研通管家采纳,获得10
10秒前
共享精神应助科研通管家采纳,获得10
10秒前
10秒前
情怀应助科研通管家采纳,获得10
10秒前
李爱国应助科研通管家采纳,获得10
10秒前
所所应助科研通管家采纳,获得10
10秒前
10秒前
Orange应助科研通管家采纳,获得10
10秒前
10秒前
10秒前
Lucas完成签到,获得积分10
12秒前
C胖胖完成签到,获得积分10
12秒前
舒心的完成签到,获得积分10
12秒前
zz完成签到,获得积分10
13秒前
14秒前
14秒前
14秒前
15秒前
luozejun完成签到,获得积分10
15秒前
ycp完成签到,获得积分10
16秒前
dawang完成签到 ,获得积分10
16秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029