表面等离子共振
信号(编程语言)
材料科学
检出限
光纤
灵敏度(控制系统)
再现性
重复性
等离子体子
光纤传感器
纳米技术
光电子学
计算机科学
光学
化学
纳米颗粒
物理
电子工程
色谱法
工程类
程序设计语言
作者
Wei Zhang,Hong Lei,J. Li,Xiaoyan Wang,Hui Yan
标识
DOI:10.1016/j.snb.2023.135004
摘要
MicroRNAs are important tumor biomarkers. How to effectively detect them by sensors is a challenge because of their extremely low concentrations. We proposed a dual signal enhancement strategy to develop the fiber optic (FO) localized surface plasmon resonance (LSPR) sensor for the highly sensitive detection of miR-103. It included two stages, one was to obtain the hot-spot by constructing the multimer structure, AuNP-spacer- AuNPs, and another was the combination of capturing miR-103 by the probe and catalytic amplification by Hexagonal star G-quadruplex catalyzing 4-chloro-1-naphthol to generate benzo-4-chlorohexadienone, which deposited on the AuNPs, resulting in the generation of LSPR peak redshift. The parameters of the preparation and detection application were systematically optimized. The redshift values had a good linear relationship with the logarithms of miR-103 concentrations, with high R2 0.9973, wide detection range 102 - 107 fM, and low LOD 6.4 fM. The method had high specificity, repeatability, and reproducibility, and was also successfully validated with real samples. Therefore, the dual signal strategy for constructing FO LSPR sensors can achieve the high sensitivity detection of extremely low concentration of MicroRNAs, which will have great application prospects in medicine and life science.
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