生物正交化学
超分子化学
化学
广谱
纳米技术
计算机科学
组合化学
材料科学
分子
点击化学
有机化学
作者
Ranjan Sasmal,Arka Som,Pratibha Kumari,Resmi V. Nair,Sushanta Show,Nisha Sanjay Barge,Meenakshi Pahwa,Nilanjana Das Saha,Sushma Rao,Vasu Sheeba,Rachit Agarwal,Sarit S. Agasti
出处
期刊:ACS central science
[American Chemical Society]
日期:2024-10-08
卷期号:10 (10): 1945-1959
标识
DOI:10.1021/acscentsci.4c01080
摘要
Fluorogenic probes that unmask fluorescence signals in response to bioorthogonal reactions are a powerful new addition to biological imaging. They can significantly reduce background fluorescence and minimize nonspecific signals, potentially enabling real-time, high-contrast imaging without the need to wash out excess fluorophores. While diverse classes of highly refined synthetic fluorophores are now readily available, integrating them into a bioorthogonal fluorogenic scheme still requires extensive design efforts and customized structural alterations to optimize quenching mechanisms for each specific fluorophore scaffold. Herein, we present a highly generalizable strategy that can produce an efficient bioorthogonal fluorogenic response from essentially any readily available fluorophore without further structural alterations. We designed this strategy based on the macrocyclic cucurbit[7]uril (CB7) host, where a fluorogenic response is achieved by programming a guest exchange reaction within the macrocyclic cavity. We employed this strategy to rapidly create fluorogenic probes across the visible spectrum from diverse fluorophore scaffolds, which enabled no-wash imaging in live cells and tissues with minimal background signal. Finally, we demonstrated that this strategy can be combined with metabolic labeling for fluorogenic detection of metabolically tagged mycobacteria under no-wash conditions and paired with covalently clickable probes for high-contrast super-resolution and multiplexed imaging in cells and tissues.
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