丝素
旁分泌信号
细胞生物学
间充质干细胞
干细胞
再生(生物学)
丝胶
再生医学
伤口愈合
组织工程
化学
生物
丝绸
免疫学
生物化学
材料科学
受体
遗传学
复合材料
作者
Yanan Zhang,Renwang Sheng,Jialin Chen,Hongmei Wang,Yue Zhu,Zhicheng Cao,Xinyi Zhao,Zhimei Wang,Chuanquan Liu,Zhixuan Chen,Po Zhang,Baian Kuang,Haotian Zheng,Qingqiang Yao,Wei Zhang
标识
DOI:10.1101/2022.09.29.510013
摘要
Abstract Silk fibroin (SF) and sericin (SS), the two major proteins of silk, are attractive biomaterials that show great potential in regenerative medicine. However, their biochemical interactions with stem cells were not fully understood. Here, we employed multiomics to obtain a global view of the triggered cellular processes and pathways of MSCs by SF and SS. Integrated RNA-seq and proteomics revealed that SF and SS strongly enhanced the paracrine activity of MSCs through differentially activating integrin and glycolytic pathways, rather than directly regulating stem cell fate to initiate multiple but distinct biological processes in MSCs. Those specific paracrine signals of MSCs stimulated by SF and SS effectively promoted skin wound healing by influencing the behaviors of multiple resident cells in skin wound microenvironments. This study provides comprehensive and reliable insights into the cellular interactions with SF and SS, enabling future development of silk-based therapeutics for tissue engineering and stem cell therapy.
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