化学
色谱法
固相萃取
醋酸铵
高效液相色谱法
洗脱
萃取(化学)
分析物
小檗碱
甲醇
有机化学
作者
Yayun Yang,Lvye Liu,Zhengtao Wang,Yan Jin,Yanhai Zhang
标识
DOI:10.1080/00032719.2022.2125525
摘要
An automated on-line solid-phase extraction (SPE) high-performance liquid chromatography (HPLC) method was developed for the determination of six protoberberine alkaloids in compound Chinese medicines containing Coptidis Rhizoma and Phellodendri Cortex. The sample solution extracted by acidic-methanol was loaded into the constructed system directly to perform enrichment and the purification of the analytes was performed automatically. A cation-exchanged sorbent based on turbulent chromatography was employed for on-line SPE to remove macro and neutral matrix interferences from samples. The target alkaloids were retained on the SPE column and eluted onto the trapping C18 column by a mobile phase of methanol/500 mM ammonium acetate (85:15) at a flow rate of 0.3 mL/min. Ion pair reverse phase high-performance liquid chromatography was employed to complete the determination of six analytes. The results showed the linear correlation coefficients for all analytes were above 0.999. The peak area of relative standard deviation in reproducibility measurements were from 0.55% to 2.39%. Recovery measurements spiked at two levels were from 92.71% to 104.28%. The reported method was suitable for the determination of six active alkaloids in multicomponent Chinese medicines containing Coptidis rhizoma and Phellodendri cortex.
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