抵抗性
肥料
肥料
土壤水分
生物
厚壁菌
堆肥
基因组
微生物种群生物学
土壤微生物学
放线菌门
蛋白质细菌
农学
生态学
抗生素耐药性
抗生素
16S核糖体RNA
微生物学
细菌
基因
生物化学
整合子
遗传学
作者
Tingting Li,Ruochen Li,Yifan Cao,Chengyuan Tao,Xuhui Deng,Yannan Ou,Hongjun Liu,Zongzhuan Shen,Rong Li,Qirong Shen
标识
DOI:10.1016/j.jhazmat.2022.129704
摘要
The effects of different fertilization on microbial communities and resistome in agricultural soils with a history of fresh manure application remains largely unclear. Here, soil antibiotic resistance genes (ARGs), mobile genetic elements (MGEs) and microbial communities were deciphered using metagenomics approach from a long-term field experiment with different fertilizer inputs. A total of 541 ARG subtypes were identified, with Multidrug, Macrolides-Lincosamides-Streptogramins (MLS), and Bacitracin resistance genes as the most universal ARG types. The abundance of ARGs detected in manure (2.52 ARGs/16 S rRNA) treated soils was higher than chemical fertilizer (2.42 ARGs/16 S rRNA) or compost (2.37 ARGs/16 S rRNA) amended soils. The higher abundance of MGEs and the enrichment of Proteobacteria were observed in manure treated soils than in chemical fertilizer or compost amended soils. Proteobacter and Actinobacter were recognized as the main potential hosts of ARGs revealed by network analysis. Further soil pH was identified as the key driver in determining the composition of both microbial community and resistome. The present study investigated the mechanisms driving the microbial community, MGEs and ARG profiles of long-term fertilized soils with ARGs contamination, and our findings could support strategies to manage the dissemination of soil ARGs.
科研通智能强力驱动
Strongly Powered by AbleSci AI