免疫印迹
免疫组织化学
血管生成
信号转导
氧化应激
污渍
缺血
免疫荧光
细胞生物学
药理学
炎症
下调和上调
化学
类黄酮
医学
生物
病理
癌症研究
生物化学
免疫学
抗氧化剂
内科学
抗体
基因
作者
Yao Li,Yurun Zhu,Fei Hu,Lue Liu,Guangjie Shen,Qiming Tu
摘要
Random-pattern skin flaps have been widely used in the reconstruction of damaged tissues. Ischemia-reperfusion injury occurring in the distal regions of the flap is a common issue, which often leads to flap necrosis and restricts its clinical applications. Procyanidin B2 (PB2), a naturally occurring flavonoid in large quantities in various fruits, has been demonstrated to exhibit several significant pharmacological properties. However, the effect of PB2 on flap viability is not clearly known. Here, using Western blot analysis, immunohistochemistry, and immunofluorescence staining, we observed that PB2 significantly reduced oxidative stress and inflammation and enhanced angiogenesis. Mechanically, we provided evidence for the first time that the beneficial effects of PB2 occur through the activation of the Sirt1/Nrf2 signaling pathway. Moreover, co-administration of PB2 and EX527, a selective inhibitor of Sirt1, resulted in down-regulation of the expression of Sirt1, Nrf2, and downstream antioxidants. In summary, our study showed that PB2 might be a novel therapeutic strategy for improving the survival of random-pattern skin flaps.
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