自体荧光
荧光
粒子(生态学)
材料科学
生物物理学
荧光寿命成像显微镜
纳米技术
光学
生物
物理
生态学
作者
Ikeda Trashi,Mateusz Z. Durbacz,Orikeda Trashi,Yalini H. Wijesundara,Ryanne N. Ehrman,Alyssa C. Chiev,Cary B. Darwin,Fabian C. Herbert,Jashkaran Gadhvi,Nicole J. De Nisco,Steven O. Nielsen,Jeremiah J. Gassensmith
摘要
Virus-like particles (VLPs) are engineered nanoparticles that mimic the properties of viruses-like high tolerance to heat and proteases-but lack a viral genome, making them non-infectious. They are easily modified chemically and genetically, making them useful in drug delivery, enhancing vaccine efficacy, gene delivery, and cancer immunotherapy. One such VLP is Qβ, which has an affinity towards an RNA hairpin structure found in its viral RNA that drives the self-assembly of the capsid. It is possible to usurp the native way infectious Qβ self-assembles to encapsidate its RNA to place enzymes inside the VLP's lumen as a protease-resistant cage. Further, using RNA templates that mimic the natural self-assembly of the native capsid, fluorescent proteins (FPs) have been placed inside VLPs in a "one pot" expression system. Autofluorescence in tissues can lead to misinterpretation of results and unreliable science, so we created a single-pot expression system that uses the fluorescent protein smURFP, which avoids autofluorescence and has spectral properties compatible with standard commercial filter sets on confocal microscopes. In this work, we were able to simplify the existing "one-pot" expression system while creating high-yielding fluorescent VLP nanoparticles that could easily be imaged inside lung epithelial tissue.
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