Sulforaphane alleviated vascular remodeling in hypoxic pulmonary hypertension via inhibiting inflammation and oxidative stress

莱菔硫烷 氧化应激 缺氧(环境) SOD2 肺动脉高压 细胞凋亡 炎症 超氧化物歧化酶 丙二醛 医学 化学 右心室肥大 谷胱甘肽 癌症研究 内科学 内分泌学 药理学 生物化学 有机化学 氧气
作者
Jinjin Pan,Rui Wang,Yandong Pei,Dingyou Wang,Na Wu,Yuke Ji,Qing Tang,Liang Liu,Ke Cheng,Qiwang Liu,Jian Sun,Miaomiao Gong,Xu Zheng,Junxia Li,Changlin Zhang,Yuhui Yuan
出处
期刊:Journal of Nutritional Biochemistry [Elsevier]
卷期号:111: 109182-109182 被引量:18
标识
DOI:10.1016/j.jnutbio.2022.109182
摘要

Hypoxic pulmonary hypertension (HPH) is a cardiopulmonary disease featured by pulmonary vascular remodeling, which is due to abnormal proliferation of pulmonary artery smooth muscle cells (PASMCs) and dysfunction of endothelial cells (ECs). Sulforaphane (SFN) is a natural isothiocyanate extracted from cruciferous vegetables with promising anti-inflammatory and anti-oxidative activities. This study aimed to explore the effect and mechanism of SFN on HPH. Male mice were exposed to persistent chronic hypoxia for 4 weeks to induce HPH. The results demonstrated that SFN repressed the increased right ventricular systolic pressure (RVSP) and attenuated the right ventricular hypertrophy and pulmonary arteries remodeling in HPH mice. In particular, after SFN treatment, the CD68 positive cells in lung sections were reduced; TNF-α and IL-6 levels in lungs and serum declined; activation of NF-κB in PASMCs was inhibited in response to hypoxia. Besides, SFN enhanced the superoxide dismutase (SOD) activity in serum, SOD2 expression, total glutathione levels, and GSH/GSSG ratio in PASMCs, along with a decrease in malondialdehyde (MDA) contents in serum and ROS production in PASMCs after hypoxia exposure. Notably, SFN, as an Nrf2 activator, reversed the reduction in Nrf2 expression in hypoxic PASMCs. In vitro, SFN treatment inhibited hyperproliferation and promoted apoptosis of PASMCs under hypoxia conditions. SFN also prevented the apoptosis of pulmonary microvascular ECs caused by hypoxia. Therefore, these data suggested that SFN could significantly restrain the inflammation and oxidative stress, thereby inhibiting PASMCs proliferation, promoting PASMCs apoptosis, and reversing hypoxia injury in ECs to improve pulmonary vascular remodeling.
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