断点群集区域
B细胞
细胞生长
细胞生物学
生物
淋巴细胞生成
B细胞受体
信号转导
细胞
分子生物学
受体
造血
干细胞
免疫学
生物化学
遗传学
抗体
作者
Shouxin Zhang,Dongya Cui,Miaohui Huang,Yongwei Zheng,Baijiao Zheng,Li‐Ling Chen,Qi Chen
标识
DOI:10.1096/fj.202201909rr
摘要
Abstract The paraspeckle protein NONO is a multifunctional nuclear protein participating in the regulation of transcriptional regulation, mRNA splicing and DNA repair. However, whether NONO plays a role in lymphopoiesis is not known. In this study, we generated mice with global deletion of NONO and bone marrow (BM) chimeric mice in which NONO is deleted in all of mature B cells. We found that the global deletion of NONO in mice did not affect T‐cell development but impaired early B‐cell development in BM at pro‐ to pre‐B‐cell transition stage and B‐cell maturation in the spleen. Studies of BM chimeric mice demonstrated that the impaired B‐cell development in NONO‐deficient mice is B‐cell‐intrinsic. NONO‐deficient B cells displayed normal BCR‐induced cell proliferation but increased BCR‐induced cell apoptosis. Moreover, we found that NONO deficiency impaired BCR‐induced activation of ERK, AKT, and NF‐κB pathways in B cells, and altered BCR‐induced gene expression profile. Thus, NONO plays a critical role in B‐cell development and BCR‐induced B‐cell activation.
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