生物
骨骼肌
细胞生物学
基因表达谱
基因剔除小鼠
长非编码RNA
核糖核酸
计算生物学
基因表达
遗传学
基因
解剖
作者
Mingming Chen,Di Lian,Yan Li,Yue Zhao,Xueling Xu,Zhimei Liu,Jinlong Zhang,Xiaosheng Zhang,S. Vincent Wu,Shaohua Qi,Shoulong Deng,Kun Yu,Zhengxing Lian
标识
DOI:10.1089/dna.2022.0574
摘要
Improving livestock and poultry growth rates and increasing meat production are urgently needed worldwide. Previously, we produced a myostatin (MSTN) and fibroblast growth factor 5 (FGF5) double-knockout (MF−/−) sheep by CRISPR Cas9 system to improve meat production, and also wool production. Both MF−/− sheep and the F1 generation (MF+/−) sheep showed an obvious “double-muscle” phenotype. In this study, we identified the expression profiles of long noncoding RNAs (lncRNAs) in wild-type and MF+/− sheep, then screened out the key candidate lncRNAs that can regulate myogenic differentiation and skeletal muscle development. These key candidate lncRNAs can serve as critical gatekeepers for muscle contraction, calcium ion transport and skeletal muscle cell differentiation, apoptosis, autophagy, and skeletal muscle inflammation, further revealing that lncRNAs play crucial roles in regulating muscle phenotype in MF+/− sheep. In conclusion, our newly identified lncRNAs may emerge as novel molecules for muscle development or muscle disease and provide a new reference for MSTN-mediated regulation of skeletal muscle development.
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