Formation characteristics of algal-bacteria granular sludge under low-light environment: From sludge characteristics, extracellular polymeric substances to microbial community

In this study, the formation characteristics of algal-bacteria granular sludge (ABGS) under low-light environment (80, 110, and 140 μmol/m2/s) were investigated. The findings revealed that the stronger light intensity favored the improvement of sludge characteristics, nutrient removal performances, and extracellular polymeric substance (EPS) secretion at the growing stage, which were more preferential to facilitate the formation of ABGS. However, after the mature stage, the weaker light intensity ensured more stable operation of the system, as shown by contributing to sludge settlement performance, denitrification, and EPS secretion. According to the results of high-throughput sequencing, the dominant bacterial genus of the mature ABGS cultured under low light intensity were all Zoogloe, while the dominant algal genus was different. For the mature ABGS, the 140 and 80 μmol/m2/s light intensity had the most significant activation effect to the functional genes related to carbohydrate metabolism and amino acid metabolism, respectively.