Obtaining an Array of Closely Packed Mesoscale Spheres Using Dielectrophoresis Forces for Optical Super-Resolution Microscopy

We reported on the proof of the concept of dielectric mesoscale particle array-based cover glass for optical super-resolution microscopy. The limited field of view is a limitation of ultra-high resolution microscopy based on single mesoscale particles. Using the forces of dielectrophoresis, we placed an ordered mesoscale spheres array over the sample and overcame this limitation using. A dielectrophoresis force acted on the mesoscale sphere electric dipole, which has set the particle into the forward motion due to an induced dipole moment formed in the volume of such particle placed in an inhomogeneous alternating electric field. The latex spheres were placed into the gap between the electrodes of the measuring cell. After the application of an electric field, the microspheres were grouped into arrays of densely spaced particles. The preliminary experiments confirmed the possibility of organizing an array of densely packed mesoscale particles under the action of dielectrophoresis force. The results obtained can be used for various applications in ultra-high resolution microscopy with extended functionality.