粉虱
食品科学
塔克曼
生物
食物过敏原
食品加工
化学
实时聚合酶链反应
过敏原
生物化学
植物
基因
过敏
免疫学
幼虫
作者
Caterina Villa,Mónica B. M. V. Moura,Carla S. Silva Teixeira,Joana Costa,Isabel Mafra
出处
期刊:Nutrients
[MDPI AG]
日期:2023-01-17
卷期号:15 (3): 482-482
被引量:5
摘要
The consumption of insects has increased in western countries, raising concerns about their potential to induce food allergic reactions in sensitized/allergic individuals. This work intended to develop a real-time PCR approach for the detection/quantification of yellow mealworm (Tenebrio molitor) as a potential allergenic food in complex matrices. For this purpose, reference mixtures simulating the production of pork sausages and wheat biscuits containing known amounts of mealworm were used. Real-time PCR with TaqMan probe targeting the cytochrome b gene of T. molitor was able to detect up to 2 fg of insect DNA, and 1.0 and 0.1 mg/kg of mealworm flour in autoclaved sausages and baked biscuits, respectively. Generally, the method showed acceptable analytical performance parameters, confirming its suitability/applicability for a wide range of foods. However, real-time PCR data showed significant differences among food matrix and processing, highlighting the importance of using appropriate calibration models for quantitative analysis. Finally, the real-time PCR approach was successfully validated with blind mixtures and applied to commercial samples, demonstrating its efficacy and reliability in the quantification of mealworm in processed foodstuffs.
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