亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Protective effects of KLF4 on blood–brain barrier and oxidative stress after cerebral ischemia–reperfusion in rats through the Nrf2/Trx1 pathway

体内 氧化应激 星形胶质细胞 药理学 血脑屏障 标记法 免疫印迹 KLF4公司 缺血 化学 埃文斯蓝 细胞凋亡 医学 生物 生物化学 中枢神经系统 内分泌学 内科学 转录因子 SOX2 生物技术 基因
作者
Tao Huang,Junping Yin,Song'e Ren,Xuling Zhang
出处
期刊:Cytokine [Elsevier]
卷期号:169: 156288-156288 被引量:10
标识
DOI:10.1016/j.cyto.2023.156288
摘要

To investigate the role of KLF4 in CI/R injury and whether Nrf2/Trx1 axis acted as a downstream pathway of KLF4 to exert the protective role in blood–brain barrier destruction after CI/R. The tMCAO rat model in vivo was constructed and received the intracerebroventricular injection of 5 μg/kg and 10 μg/kg rhKLF4 before operation. TTC, brain water content, neurological function, ELISA, behavioral tests, HE, TUNEL, and qRT-PCR were performed to detect the protective role of KLF4 on CIR. Double-fluorescence staining and western blot were performed to determine the localization and spatiotemporal expression in brain tissues. Furthermore, we also analyzed the effect of KLF4 on the blood–brain barrier (BBB) and related mechanisms in vivo and in vitro. Nrf2 inhibitor tretinoin was applied, which was intraperitoneally injected into CIR rat. Evans blue staining was conducted. In vitro OGD/R models of bEnd.3 cells were also established, and received KLF4 overexpressed transfection and 12.5 µM tretinoin incubation. The permeability of bEnd.3 cells was evaluated by TEER and FITC-dextran leakage. BBB-related factors and oxidative stress were also analyzed, respectively. The tubular ability of KLF4 on OGD/R bEnd3 cells was also evaluated. In vivo study confirmed that KLF4 was expressed in astrocyte, and its content increased with time. KLF4 protected against brain injury caused by cerebral ischemia–reperfusion, reduced cerebral infarction area and oxidative stress levels, and promoted the recovery of behavioral ability in rats. Simultaneously, mechanism experiments confirmed that the repair effect of KLF4 on cerebral ischemia–reperfusion injury was closely related to the Nrf2/Trx1 pathway. KLF4 exerted the neuroprotective effect through upregulating Nrf2/Trx1 pathway. Consistent with in vivo animal study, in vitro study also confirmed the effect of KLF4 on the permeability of bEnd.3 cells after OGD/R injury through Nrf2/Trx1 pathway. Collectively, KLF4 played neuroprotective role in CIR induced MCAO and OGD/R, and the beneficial effects of KLF4 was partly linked to Nrf2/Trx1 pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
活力鸿发布了新的文献求助10
9秒前
思源应助科研通管家采纳,获得10
19秒前
可久斯基完成签到 ,获得积分10
30秒前
爆米花应助wenwen采纳,获得10
50秒前
1分钟前
wenwen发布了新的文献求助10
1分钟前
1分钟前
wenwen完成签到,获得积分10
1分钟前
xiaolang2004完成签到,获得积分10
1分钟前
1分钟前
等待香寒完成签到 ,获得积分10
2分钟前
蛋白积聚完成签到,获得积分10
2分钟前
zombleq完成签到 ,获得积分10
2分钟前
迷你的靖雁完成签到,获得积分10
3分钟前
Orange应助威武谷南采纳,获得10
3分钟前
taotao发布了新的文献求助10
3分钟前
3分钟前
3分钟前
威武谷南发布了新的文献求助10
3分钟前
3分钟前
SoftwarePrince完成签到,获得积分10
4分钟前
郗妫完成签到,获得积分10
4分钟前
jyy完成签到,获得积分10
4分钟前
4分钟前
4分钟前
4分钟前
spark810完成签到,获得积分0
5分钟前
factor发布了新的文献求助10
5分钟前
彭于晏应助欢呼的寻双采纳,获得10
5分钟前
激动的似狮完成签到,获得积分10
5分钟前
完美世界应助一杯美式采纳,获得10
5分钟前
5分钟前
一杯美式发布了新的文献求助10
6分钟前
6分钟前
6分钟前
7分钟前
英俊的铭应助iris采纳,获得10
7分钟前
领导范儿应助大爷醒醒啊采纳,获得10
7分钟前
7分钟前
iris发布了新的文献求助10
7分钟前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137011
求助须知:如何正确求助?哪些是违规求助? 2787960
关于积分的说明 7784128
捐赠科研通 2444060
什么是DOI,文献DOI怎么找? 1299643
科研通“疑难数据库(出版商)”最低求助积分说明 625497
版权声明 600989