In vitro evaluation of probiotic properties and selenium bioaccumulation of lactic acid bacteria isolated from poultry gastrointestinal, as an organic selenium source

动物双歧杆菌 益生菌 微生物学 嗜酸乳杆菌 罗伊乳杆菌 生物累积 生物 食品科学 乳酸菌 化学 细菌 双歧杆菌 发酵 生态学 遗传学
作者
Shokoufeh Norouzi,Mohsen Daneshyar,Parviz Farhoomand,Amir Tukmechi,Guillermo Tellez-Isaiasc
出处
期刊:Research in Veterinary Science [Elsevier BV]
卷期号:162: 104934-104934 被引量:1
标识
DOI:10.1016/j.rvsc.2023.06.012
摘要

The purpose of this study was to examine the probiotic characteristics and selenium (Se) bioaccumulation potential of five Lactobacillus strains in vitro. Lactobacillus acidophilus, L. delbrueckii subsp. lactis, L. reuteri, L. gallinarum, and L. animalis were among the strains employed. As significant aspects of probiotics, identification, and evaluation of their survival potential in the gastrointestinal system were undertaken. Although all experimental Lactobacillus strains bioaccumulated Se (IV) concentrations in media culture, three Lactobacillus strains (L. animalis, L. gallinarum, and L. acidophilus) bioaccumulated the highest Se concentrations (23.08, 8.62, and 8.51 mg/g, respectively) after culture in the presence of 1.5 mg/ml sodium selenite. By disc diffusion, all isolates were evaluated for antibiotic susceptibility against six antibiotics, including ciprofloxacin, ampicillin, methicillin, streptomycin, tetracycline, and trimethoprim-sulfamethoxazole. Many of the isolates tested positive for resistance to some of the antibiotics utilized. The L. reuteri and L. gallinarum were found to be resistant to about 50% of the antibiotics that were tested. In terms of acid tolerance, L. animalis showed significant resistance at acidic pH by 1.72 log unit reduction whereas L. delbrueckii and L. galliinarum showed significant sensitivity at acidic pH (P > 0.05). Bile tolerance was addressed as an important aspect of the safety assessment for probiotics. There were variances in acid and bile tolerance among species, although all of them tolerated stress conditions to an acceptable degree. Upon comparing the various species, it was observed that L. gallinarum exhibited a significant decline in growth, as evidenced by a decrease of 1.39 log units in cell viability. On the other hand, L. acidophilus and L. animalis demonstrated remarkable bile tolerance, with 0.09 and 0.23 log unit reduction respectively (P < 0.05). These results suggest that L. animalis, L. gallinarum, and L. acidophilus, can be good candidates to evaluate them in vivo in further investigations due to their tolerance to acid, and bile, antibiotic resistance, and strong ability to bioaccumulate Se in chickens.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Jasper应助大橙子采纳,获得10
刚刚
GUO发布了新的文献求助30
1秒前
三石完成签到 ,获得积分10
1秒前
跳跃的白云完成签到 ,获得积分10
2秒前
酷酷亦寒完成签到 ,获得积分10
4秒前
量子星尘发布了新的文献求助10
6秒前
Blaseaka完成签到 ,获得积分10
7秒前
Owen应助科研通管家采纳,获得10
7秒前
深情安青应助科研通管家采纳,获得10
7秒前
7秒前
汉堡包应助科研通管家采纳,获得10
8秒前
小二郎应助科研通管家采纳,获得30
8秒前
爆米花应助科研通管家采纳,获得10
8秒前
沉静的浩然完成签到,获得积分10
9秒前
开朗的绮山完成签到,获得积分10
9秒前
9秒前
老迟到的土豆完成签到 ,获得积分10
12秒前
单薄的日记本完成签到,获得积分10
12秒前
大橙子发布了新的文献求助10
13秒前
舒适的天奇完成签到 ,获得积分10
14秒前
15秒前
17秒前
都是小儿卡通书完成签到,获得积分10
18秒前
19秒前
陶醉的又夏完成签到 ,获得积分10
19秒前
lily完成签到 ,获得积分10
22秒前
23秒前
子苓完成签到 ,获得积分10
25秒前
Jun完成签到 ,获得积分10
25秒前
phil完成签到,获得积分10
26秒前
祁乐安发布了新的文献求助20
26秒前
如初完成签到,获得积分10
27秒前
zzuwxj完成签到,获得积分10
30秒前
糊涂的语兰完成签到,获得积分10
34秒前
多余完成签到,获得积分10
35秒前
喝酸奶不舔盖完成签到 ,获得积分10
35秒前
朴实初夏完成签到 ,获得积分10
36秒前
36秒前
华仔应助查查make采纳,获得10
41秒前
乐乐应助普鲁卡因采纳,获得10
41秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038157
求助须知:如何正确求助?哪些是违规求助? 3575869
关于积分的说明 11373842
捐赠科研通 3305650
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022