In vitro evaluation of probiotic properties and selenium bioaccumulation of lactic acid bacteria isolated from poultry gastrointestinal, as an organic selenium source

动物双歧杆菌 益生菌 微生物学 嗜酸乳杆菌 罗伊乳杆菌 生物累积 生物 食品科学 乳酸菌 化学 细菌 双歧杆菌 发酵 生态学 遗传学
作者
Shokoufeh Norouzi,Mohsen Daneshyar,Parviz Farhoomand,Amir Tukmechi,Guillermo Tellez-Isaiasc
出处
期刊:Research in Veterinary Science [Elsevier BV]
卷期号:162: 104934-104934 被引量:1
标识
DOI:10.1016/j.rvsc.2023.06.012
摘要

The purpose of this study was to examine the probiotic characteristics and selenium (Se) bioaccumulation potential of five Lactobacillus strains in vitro. Lactobacillus acidophilus, L. delbrueckii subsp. lactis, L. reuteri, L. gallinarum, and L. animalis were among the strains employed. As significant aspects of probiotics, identification, and evaluation of their survival potential in the gastrointestinal system were undertaken. Although all experimental Lactobacillus strains bioaccumulated Se (IV) concentrations in media culture, three Lactobacillus strains (L. animalis, L. gallinarum, and L. acidophilus) bioaccumulated the highest Se concentrations (23.08, 8.62, and 8.51 mg/g, respectively) after culture in the presence of 1.5 mg/ml sodium selenite. By disc diffusion, all isolates were evaluated for antibiotic susceptibility against six antibiotics, including ciprofloxacin, ampicillin, methicillin, streptomycin, tetracycline, and trimethoprim-sulfamethoxazole. Many of the isolates tested positive for resistance to some of the antibiotics utilized. The L. reuteri and L. gallinarum were found to be resistant to about 50% of the antibiotics that were tested. In terms of acid tolerance, L. animalis showed significant resistance at acidic pH by 1.72 log unit reduction whereas L. delbrueckii and L. galliinarum showed significant sensitivity at acidic pH (P > 0.05). Bile tolerance was addressed as an important aspect of the safety assessment for probiotics. There were variances in acid and bile tolerance among species, although all of them tolerated stress conditions to an acceptable degree. Upon comparing the various species, it was observed that L. gallinarum exhibited a significant decline in growth, as evidenced by a decrease of 1.39 log units in cell viability. On the other hand, L. acidophilus and L. animalis demonstrated remarkable bile tolerance, with 0.09 and 0.23 log unit reduction respectively (P < 0.05). These results suggest that L. animalis, L. gallinarum, and L. acidophilus, can be good candidates to evaluate them in vivo in further investigations due to their tolerance to acid, and bile, antibiotic resistance, and strong ability to bioaccumulate Se in chickens.
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