适体
食管鳞状细胞癌
食管癌
指数富集配体系统进化
癌症研究
肿瘤科
鳞状细胞癌
细胞
细胞培养
DNA
基底细胞
癌症
医学
内科学
生物
分子生物学
遗传学
基因
核糖核酸
作者
Baijiang Jin,Gaojian Yang,Zhukang Guo,Zhu Chen,Yuan Liu,Li Song,Hui Chen,Yile Fang,Yan Deng,Nongyue He
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2024-01-01
卷期号:16 (28): 4683-4690
摘要
Esophageal cancer is a common cancer with high morbidity and mortality that severely threatens the safety and quality of human life. The strong metastatic nature of esophageal cancer enables it to metastasize more quickly and covertly, making it difficult for current diagnostic and treatment methods to achieve efficient early screening, as well as timely and effective treatment. As a promising solution, nucleic acid aptamers, a kind of special single-stranded DNA or RNA oligonucleotide selected by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology, can specifically bind with different molecular targets. In this paper, random DNA single-stranded oligonucleotides were used as the initial library. Using TE-1 cells and HEEC cells as targets, specific binding sequences were selected by 15 rounds of the cell-SELEX method, and the aptamer sequence that binds to TE-1 cells with the most specificity was obtained and named Te4. The Te4 aptamer was further validated for binding specificity, binding affinity, type of target,
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