倍他林
报告基因
基因表达
基因
基因表达调控
生物
转录因子
遗传学
计算生物学
化学
有机化学
颜料
作者
Jia Liu,Hao Li,Conghao Hong,Wanqing Lu,Wei Zhang,Hongbo Gao
摘要
Abstract Various reporter genes have been developed to study gene expression pattern and gene regulation. The RUBY reporter gene was recently developed and widely used, because of its visible and noninvasive advantages. However, quantitative analysis of RUBY gene expression levels was lacking. In this study, we introduce a novel betalain quantification method in combination with the tobacco transient expression system. The betalain produced in tobacco leaves was extracted and purified, and its concentration was quantitatively measured. We successfully applied this approach in studying the transcriptional regulation of ARC5 gene by transcription factors CPD25 and CPD45. Furthermore, with this method, we showed that the gene expression of RCA and Rbcs1A gene were regulated by light, transcription factors HY5 and PIFs through G‐box and I‐box elements. The development of this betalain quantification approach with the tobacco transient expression system offers a cost‐effective and intuitive strategy for studying the regulatory mechanism of gene expression.
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