化学发光
抗生素
微生物学
内酰胺
抗生素耐药性
生物
医学
化学
色谱法
立体化学
作者
Zheng Ma,Runqiu Liu,Jie Wang,Tao Yu,Yingqiu Zou,Fangfang Chen,Cui Cui,Huixin Yang,Hexin Xie
出处
期刊:ACS Infectious Diseases
[American Chemical Society]
日期:2024-05-31
卷期号:10 (6): 1970-1979
标识
DOI:10.1021/acsinfecdis.3c00682
摘要
Bacterial resistance caused by β-lactamases has been a major threat to public health around the world, seriously weakening the efficacy of β-lactam antibiotics, the most widely used therapeutic agents against infectious diseases. To detect the bacterial resistance to β-lactam antibiotics, particularly specific type of β-lactam antibiotics, in a rapid manner, we report herein a relay-response chemiluminescence assay. This assay mainly consists of two reagents: a β-lactam-caged thiophenol and a thiophenol-sensitive chemiluminescence reporter, both of which are synthetically feasible. The selective hydrolysis of β-lactam by β-lactamase leads to the releasing of free thiophenol, which then triggers the emission of a chemiluminescence signal in a relay manner. Three thiophenol-caged β-lactams, structural analogues of cephalothin, cefotaxime, and meropenem, respectively, have been synthesized. And the application of this assay with these analogues of β-lactam antibiotics allows fast detection of β-lactamase-expressing resistant bacteria and, more impressively, provides detailed information on the resistant scope of bacteria.
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