生物
MYB公司
黄酮醇
花青素
转录因子
成熟
生物化学
拟南芥
调节基因
基因
类黄酮
类黄酮生物合成
基因表达
突变体
植物
转录组
抗氧化剂
作者
Asaph Aharoni,C. H. Ric De Vos,Martina Wein,Zhongkui Sun,Raffaella Greco,Arthur R. Kroon,Joseph N. M. Mol,Ann P. O’Connell
出处
期刊:Plant Journal
[Wiley]
日期:2001-11-01
卷期号:28 (3): 319-332
被引量:575
标识
DOI:10.1046/j.1365-313x.2001.01154.x
摘要
Fruit ripening is characterized by dramatic changes in gene expression, enzymatic activities and metabolism. Although the process of ripening has been studied extensively, we still lack valuable information on how the numerous metabolic pathways are regulated and co-ordinated. In this paper we describe the characterization of FaMYB1, a ripening regulated strawberry gene member of the MYB family of transcription factors. Flowers of transgenic tobacco lines overexpressing FaMYB1 showed a severe reduction in pigmentation. A reduction in the level of cyanidin 3-rutinoside (an anthocyanin) and of quercetin-glycosides (flavonols) was observed. Expression of late flavonoid biosynthesis genes and their enzyme activities were adversely affected by FaMYB1 overexpression. Two-hybrid assays in yeast showed that FaMYB1 could interact with other known anthocyanin regulators, but it does not act as a transcriptional activator. Interestingly, the C-terminus of FaMYB1 contains the motif pdLNL(D)/(E)Lxi(G)/S. This motif is contained in a region recently proposed to be involved in the repression of transcription by AtMYB4, an Arabidopsis MYB protein. Our results suggest that FaMYB1 may play a key role in regulating the biosynthesis of anthocyanins and flavonols in strawberry. It may act to repress transcription in order to balance the levels of anthocyanin pigments produced at the latter stages of strawberry fruit maturation, and/or to regulate metabolite levels in various branches of the flavonoid biosynthetic pathway.
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