区域选择性
酰化
化学
脂肪酶
超临界流体
催化作用
南极洲假丝酵母
超临界二氧化碳
皱纹假丝酵母
有机化学
结构异构体
酶
作者
Cleofe Palocci,Mariangela Falconi,Laura Chronopoulou,E. Cernia
标识
DOI:10.1016/j.supflu.2007.11.009
摘要
Candida rugosa lipase (CRL) was used to catalyze the regioselective acylation of methyl 6-O-trityl β-d-glucopyranoside in supercritical CO2 (SCCO2), using vinyl acetate as the acyl donor and obtained final conversion values up to 91.4%. The effect of pressure, temperature, enzyme quantity and sugar/ester molar ratio on the catalytic efficiency and regioselectivity of CRL was evaluated. The regioselectivity of the reaction was shifted towards the synthesis of 3-O-acetyl 6-O-trityl β-d-glucopyranoside and this is a function of the chemico-physical parameters of the supercritical medium employed. In fact, by varying the CO2 pressure used in the process up to 20 MPa at constant temperature, we were able to obtain the formation of the sole 3-O-acetyl 6-O-trityl β-d-glucopyranoside regioisomer. A kinetic study was also performed and the relative kinetic parameters calculated.
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