生物累积
蜡样芽孢杆菌
蜡样体
生物修复
生物吸附
流出
食品科学
环境化学
化学
吸附
生物
微生物学
核化学
细菌
遗传学
生物化学
有机化学
吸附
作者
Babita Sharma,Pratyoosh Shukla
标识
DOI:10.1016/j.jhazmat.2020.123285
摘要
This study explores the soil microorganisms for their Lead bioremediation capability. The MIC values of the six Lead resistant bacteria were evaluated, and the AAS studies of these isolates estimated their Lead accumulation percentage. The results showed that the isolate namely Bacillus cereus BPS-9 as identified based on 16S rDNA gene sequences was shown to have the highest Lead accumulation potential (79.26 %) and also selected for bioaccumulation studies. Despite the reduction in growth rate, the superoxide dismutase activity of B. cereus BPS-9 was increased with a rise in the concentration of Lead manifested through increased nitro-blue tetrazolium (NBT) reduction from 3.94 % to 77.48 %. Moreover, the biosorption capacity of B. cereus BPS-9 was 193.93 mg/g and the Langmuir isotherm model showed a value of R2 = 0.9. Furthermore, the FTIR analysis also established the role of C-H, C=C, N=N, N-H, and C-O functional groups in Lead adsorption and the SEM micrographs showed that the cells of B. cereus BPS-9 became dense, adhered and distorted after Lead adsorption. Finally, the In-silico results obtained by functional analysis through SEED viewer of the whole genome of B. cereus deciphered the presence of genes encoding heavy metal resistant proteins and transporters for the efflux of heavy metals.
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