清脆的
核酸酶
计算生物学
检出限
反式激活crRNA
化学
效应器
核糖核酸
核糖核蛋白
DNA
Cas9
生物
色谱法
基因
生物化学
作者
Jie Qiao,Siyu Lin,Wenli Sun,Lixin Ma,Yi Liu
摘要
We developed a method for the rapid and quantitative detection of the effective Cas12a RNPs by the combined use of anti-CRISPR protein AcrVA1 and stem-loop RT-qPCR, achieving a limit of detection (LOD) of 1 fM.
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