磷酸蛋白质组学
膜
计算生物学
生物
植物生物学
细胞生物学
化学
磷酸化
蛋白质磷酸化
生物化学
蛋白激酶A
植物
作者
Lin Xi,Waltraud X. Schulze,Xu Wu
出处
期刊:Methods in molecular biology
日期:2020-11-12
卷期号:: 441-451
被引量:1
标识
DOI:10.1007/978-1-0716-0880-7_22
摘要
Mass spectrometry (MS) is a powerful tool to investigate plant phosphorylation dynamics on a system-wide scale (phosphoproteomics). Plant membrane phosphoproteomics enables elucidating regulatory patterns in membranes, such as kinase-target relationships in different signaling pathways. Here, we present “ShortPhos,” an efficient and simple phosphoproteomics protocol for research on plant membrane proteins, which allows fast and efficient identification and quantification of phosphopeptides from small amounts of starting plant material and/or membrane proteins. This method improves upon the efficiency of plant membrane phosphoproteomics profiling and can be applied to the study of membrane-based signaling networks.
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