This study attempted to dye regenerated cellulose fiber fabric with fluorescent dyes in different conditions (dyeing time, temperature, pH, concentration, etc.) and investigated their effects on color strength, fluorescence intensity, and other properties of dyed fabrics. The exhaust dyeing method was applied in this experiment for cellulose based knitted fabrics. The physical properties of dyed knitted fabrics before and after treatment with fluorescent dyes were determined and evaluated. After fluorescent staining, the results showed that a large number of granular fluorescent particles were coated on the fiber surface. Higher K/ S values were obtained under conditions of a dyeing temperature of 80℃, dyeing time of 30 min, and NaCl concentration of 1 wt% than other conditions. Moreover, the fluorescence intensity of the dyed fabric was also highest at 80℃. The color strength and bursting strength of dyed knitted fabrics were increased with the increase of the concentration of fluorescein sodium. Thus, cellulose based knitted fabric dyed with fluorescent dyes exhibited excellent fluorescent properties and could be used in fluorescent clothing.