Investigation of Different Extraction Technology for Addition and Subtraction Prescription of Huoluo Xiaoling Dan by Fingerprint

Objective:To study influence of different extraction technology on chemical compositions changes from addition and subtraction prescription of Huoluo Xiaoling Dan(HLXL)by HPLC fingerprint.Method:With swertiamarin,paeoniflorin,cryptotanshinone,isoimperatorin and other seven characteristic fingerprint peaks as index,which were determined by HPLC,with YMC-Pack ODS-A column,methonal-water(gradient elution)as mobile phase,detection wavelength 210 nm,flow rate 0.8 mL·min-1,column temperature 30 ℃.Effects of different solvents(water,ethanol,acetone),extract models(ultrasound,refluxing,macerating)and mixed-extract and separated-extract on relative content of each characteristic peaks were systematically investigated.Result:Different technology extracts had significant difference on relative peak area of eleven evaluating indexes.Kinds of solvent and extraction types and order of mixing had a great influence upon extraction efficiency of chemical compositions from HLXL.Conclusion:This method was reproducible,precise,stable and could be applied to study on fingerprint of HLXL with different extraction technology.