Lignin and flavonoid content increases in Hypericum perforatum cell wall after Agrobacterium tumefaciens co-cultivation

化学 贯叶连翘 细胞壁 木质素 类黄酮 槲皮素 生物化学 食品科学 生物 抗氧化剂 药理学 有机化学
作者
Rupesh Kumar Singh,Weina Hou,Gregory Marslin,Alberto Carlos Pires Dias,Gregory Franklin
出处
期刊:Planta Medica [Thieme Medical Publishers (Germany)]
卷期号:80 (16) 被引量:8
标识
DOI:10.1055/s-0034-1394589
摘要

Hypericum perforatum (HP), a high value medicinal plant used to produce several phytopharmaceuticals, remains recalcitrant to Agrobacterium tumefaciens (AT) mediated transformation. HP recognizes AT as a potential pathogen and activates its defense response [1]. Although elicitation of HP cells with AT is known to increase its xanthone content, flavonoids remained unaffected in the soluble fraction of HP [2]. However, specific analysis of HP cell wall biomass/fractions after AT elicitation revealed an increase in lignin and cell wall bound flavonoids content, possibly as mechanism of defense against AT. Briefly, cell wall biomass was isolated from control and AT elicited HP cells via consecutive extraction with hexane, acetone, methanol and distilled water. Determination of lignin content by acetyl bromide method [3] showed a significant increase in HP cells treated with AT, implying that upon elicitation by AT, HP reinforced its cell wall as a defense response. In order to analyze their phenolic profile, the cell wall biomass was subjected to extraction by 90% methanol after alkaline hydrolysis, HCl titration and ethyl acetate extraction. HPLC analysis of the cell wall bound phenolics fraction revealed an accumulation of flavonoids (e.g. epicatechin, quercetin derivatives) in the cell walls after elicitation. Upregulation of chalcone synthase (CHS), leucoanthocyanidin dioxygenase (LDOX) and Flavone 3-hydroxilase (F3 H) were found to be responsible for this accumulation. Further, DPPH reduction and TBARS assays clearly correlated the increased production of flavonoids with improved antioxidant protection of the cell wall. Collectively, our results allow us to conclude that in addition to the increase in xanthone production [2], co-cultivation of HP cells with AT also upregulates flavonoids biosynthesis as a defense mechanism. While the xanthones enrich the soluble phenolic fraction; flavonoids are incorporated into the cell wall.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
所所应助珊珊采纳,获得10
刚刚
刚刚
pentjy完成签到,获得积分10
2秒前
2秒前
小蘑菇应助洁净的大山采纳,获得10
3秒前
5秒前
外向蜡烛完成签到,获得积分10
5秒前
himsn发布了新的文献求助10
6秒前
L77完成签到,获得积分0
6秒前
顾矜应助笔墨留香采纳,获得10
6秒前
YuhaoYan发布了新的文献求助10
7秒前
我是老大应助淡然的小萱采纳,获得50
7秒前
千柳发布了新的文献求助10
7秒前
7秒前
小勇仔发布了新的文献求助10
8秒前
Wfy完成签到,获得积分10
8秒前
keyun完成签到,获得积分10
10秒前
11秒前
CipherSage应助第八维采纳,获得10
13秒前
Simon完成签到 ,获得积分10
13秒前
珊珊发布了新的文献求助10
14秒前
研友_VZG7GZ应助小橘子采纳,获得10
15秒前
15秒前
xpdnpu完成签到,获得积分10
16秒前
笔墨留香发布了新的文献求助10
17秒前
19秒前
19秒前
大模型应助weilong采纳,获得10
20秒前
月下荷花发布了新的文献求助10
20秒前
端庄的如花完成签到 ,获得积分10
21秒前
浩怡发布了新的文献求助10
22秒前
晚风起念发布了新的文献求助10
23秒前
简墨完成签到,获得积分10
24秒前
CipherSage应助博修采纳,获得10
24秒前
24秒前
顺心醉蝶完成签到 ,获得积分10
26秒前
27秒前
乐乐应助白桃采纳,获得10
27秒前
27秒前
共享精神应助端庄的石头采纳,获得10
28秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 1000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 310
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3979704
求助须知:如何正确求助?哪些是违规求助? 3523700
关于积分的说明 11218393
捐赠科研通 3261224
什么是DOI,文献DOI怎么找? 1800490
邀请新用户注册赠送积分活动 879113
科研通“疑难数据库(出版商)”最低求助积分说明 807182