实验性自身免疫性脑脊髓炎
多发性硬化
病理
中枢神经系统
免疫组织化学
脑脊髓炎
医学
炎症
生物
神经科学
免疫学
作者
Karin Steinbach,Doron Merkler
出处
期刊:Methods in molecular biology
日期:2014-01-01
卷期号:: 189-209
被引量:5
标识
DOI:10.1007/7651_2014_110
摘要
Neuropathological techniques such as conventional and immunohistochemical staining of paraffin-embedded tissue sections are instrumental for identification and characterization of aberrations of organ architecture during human inflammatory disorders of the central nervous system (CNS) as in their animal models. Here we describe step-by-step protocols for tissue processing, sectioning, and conventional and immunohistochemical stainings to display as well as quantify CNS inflammation, demyelination, and neuronal damage in experimental autoimmune encephalomyelitis (EAE), an animal model of Multiple Sclerosis (MS).
科研通智能强力驱动
Strongly Powered by AbleSci AI