扩展器
牛磺酸
精液
低温保存
精子
运动性
男科
精子活力
彗星试验
精液扩展器
生物
DNA损伤
化学
生物化学
胚胎
氨基酸
DNA
细胞生物学
医学
聚氨酯
有机化学
作者
Filiz Kutluyer,Fatih Öğretmen,Burak Evren İnanan
出处
期刊:PubMed
日期:2016-03-11
卷期号:37 (1): 41-6
被引量:9
摘要
Amino acids, present in seminal plasma at high concentration, protect spermatozoa against cell damage during cryopreservation.Experiments were designed to analyze the effect of semen extender supplemented with taurine on post-thawed sperm motility and duration, as well as DNA damage.Extenders were supplemented with 1, 2 or 4 mM taurine. Semen samples were diluted at the ratio of 1:9 with the extenders. Diluted samples were aspirated into 0.25 ml French straws and 0.1 ml pellets. DNA damage was assessed with the comet assay after cryopreservation.The percentage and duration of sperm motility were significantly increased by taurine. Additionally, sperm motility and the motility period in pellets were higher than in straws. The best concentration of taurine was 4 mM, and the highest post-thaw motility rate (72.50 ± 3.54 %) and duration (17.50 ± 0.71 s) were obtained from the extender with 4 mM in pellets. DNA damage was decreased after taurine supplementation.Pellets could be used for goldfish sperm cryopreservation. The addition of 4 mM taurine increases the post-thaw motility and decreases DNA damage on goldfish semen.
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