琼脂糖
材料科学
肺表面活性物质
多孔性
粒径
色谱法
粒度分布
复合材料
粒子(生态学)
介孔材料
化学工程
机械强度
化学
有机化学
催化作用
工程类
地质学
海洋学
作者
Sahar Amiri,Mohammad Reza Mehrnia
标识
DOI:10.1080/02726351.2018.1455776
摘要
In this work the influence of fabrication conditions (ion strength, surfactant utilization) on the macro- (particle size distribution, PSD) and microscopic [pore size distribution (PoSD), specific surface area] structure of agarose beads was investigated. The main purpose was achieving uniform sized and porous beads with improved mechanical strength for bioseparation and chromatography applications. Therefore, PSD, PoSD, mechanical resistance, and flux through packed bed of the fabricated beads (4, 6, and 8% wt/wt) were analyzed. Based on porosimetry results, it was found that increasing ion concentration or presence of surfactant (the key variable for narrowing PSD) decreases the span value of PSD (p-value <0.05); thus, leading to a more uniform distribution. Moreover, as a result of controlling PSD, the PoSD changes from micro- to meso- and macropores in higher ion charged solutions. Furthermore, the obtained PoSD affects mechanical resistance of the prepared microspheres either as single beads or packed in a column. Mesoporous 6%-agarose beads showed the highest flux due to elevated mechanical resistance and elastic characteristic. Mesoporous 4%-beads showed the highest elasticity, and thus lower flux through column. These results demonstrate that manipulation of both macro- and microscopic characteristics of the beads should be commensurate with the intended application i.e., bioadsorption chromatography.
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