适体
微分脉冲伏安法
卡那霉素
化学
水溶液中的金属离子
生物传感器
检出限
分析物
碳纳米管
离子
分析化学(期刊)
电化学
材料科学
组合化学
纳米技术
色谱法
循环伏安法
电极
抗生素
有机化学
物理化学
生物
生物化学
遗传学
作者
Falan Li,Yemin Guo,Xiangyou Wang
标识
DOI:10.1016/j.bios.2018.04.024
摘要
A dual-target electrochemical aptasensor was developed for the simultaneous detection of multiple antibiotics based on metal ions as signal tracers and nanocomposites as signal amplification strategy. Metal ions such as Cd2+ and Pb2+ could generate distinct differential pulse voltammetry (DPV) peaks. When targets were present, kanamycin (KAN) and streptomycin (STR) as models, the KAN aptamer (KAP) and STR aptamer (STP) were released from their complementary strands, with more change of Cd2+ and Pb2+ corresponding to peak currents. At the same time, complementary strand of KAP (cKAP) and STP (cSTP) were linked with the poly (A) structure (cSTP-PolyA-cKAP) to increase their conformational freedom. Graphitized multi-walled carbon nanotubes (MWCNTGr) and carbon nanofibers-gold nanoparticles (CNFs-AuNPs) as a biosensor platform enhanced the surface area to capture a large amount of cSTP-PolyA-cKAP, thus amplifying the detection response. Under the optimal conditions, the aptasensor could detect KAN and STR as low as 74.50 pM and 36.45 pM respectively with the range from 0.1 to 100 nM and exhibited excellent selectively. Moreover, this aptasensor showed promising applications for the detection of other analytes by changing corresponding aptamers.
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