紫杉醇
脱颗粒
白三烯C4
锡克
肥大细胞
林恩
花生四烯酸5-脂氧合酶
化学
免疫球蛋白E
信号转导
免疫学
酪氨酸激酶
生物
生物化学
受体
白三烯
抗体
酶
哮喘
抗氧化剂
花生四烯酸
类黄酮
作者
Shunli Pan,Xuemei Zhao,Ning Ji,Chen Shao,Bingjie Fu,Zhe Zhang,Ran Wang,Yuling Qiu,Meihua Jin,Dexin Kong
标识
DOI:10.1016/j.intimp.2019.03.038
摘要
The aim of the present study is to investigate the anti-inflammatory and anti-allergic effects of taxifolin on mast cells and mast cell-mediated allergic reaction. We assessed the effect of taxifolin on the activation of bone marrow-derived mast cells (BMMCs) and rat basophilic leukemia (RBL)-2H3 cells induced by immunoglobulin E (IgE)/antigen (Ag), and the activation of human mast cell line (HMC-1) induced by PMA plus A23187. Taxifolin inhibited degranulation, generation of leukotriene C4 (LTC4), production of interlukin-6 (IL-6), and expression of cyclooxygenase-2 (COX-2) through blocking intracellular Ca2+ mobilization, phosphorylation of phospholipase Cγ (PLCγ) and mitogen-activated protein kinases (MAPKs), translocation of cytosolic phospholipase A2 (cPLA2) and 5-lipoxygenase (5-LO), and Akt/IKK/NF-κB pathway, in BMMC cells. Furthermore, taxifolin suppressed phosphorylation of Syk, but without effect on Fyn and Lyn. Taxifolin also inhibited activation of RBL-2H3 and HMC-1 cells via Akt/IKK/NF-κB and MAPKs/cPLA2 signal pathway. Treatment with taxifolin attenuated the mast cell-mediated passive cutaneous anaphylaxis (PCA) reaction. Our results suggest that taxifolin might become a potential drug candidate for the treatment of allergic and inflammatory diseases.
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