基因簇
链霉菌
脂肽
非核糖体肽
生物
生物合成
基因
拉伤
放线菌
链霉菌科
重组DNA
生物化学
细菌
遗传学
解剖
作者
Wilaiwan Koomsiri,Yuki Inahashi,Kantinan Leetanasaksakul,Kazuro Shiomi,Yo Takahashi,Satoshi O ̄mura,Markiyan Samborskyy,Peter F. Leadlay,Pakorn Wattana-Amorn,Arinthip Thamchaipenet,Takuji Nakashima
标识
DOI:10.1021/acs.jnatprod.9b00074
摘要
Whole genome analysis of Streptomyces sp. KO-7888 has revealed various pathway-specific transcriptional regulatory genes associated with silent biosynthetic gene clusters. A Streptomyces antibiotic regulatory protein gene, speR, located adjacent to a novel nonribosomal peptide synthetase (NRPS) gene cluster, was overexpressed in the wild-type strain. The resulting recombinant strain of Streptomyces sp. KO-7888 produced two new lipopeptides, sarpeptins A and B. Their structures were elucidated by high-resolution electrospray ionization mass spectrometry, NMR analysis, and the advanced Marfey's method. The distinct modular sections of the corresponding NRPS biosynthetic gene cluster were characterized, and the assembly line for production of the lipopeptide chain was proposed.
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