Biosynthesis of large-sized silver nanoparticles using Angelica keiskei extract and its antibacterial activity and mechanisms investigation

This study demonstrates a novel approach for synthesizing large-sized silver nanoparticles (AgNPs) using Angelica keiskei water extract. The AgNPs exhibited maximum absorbance at 438 nm and were mostly spherical in shape, with an average particle size of 130.1 ± 2.1 nm. The bacteriostatic and bactericidal effects of AgNO3 against the food-borne pathogen Listeria monocytogenes were explored by measuring the minimum inhibitory concentration and minimum bactericidal concentration. The antibacterial activity mechanism of the AgNPs was further investigated. The results show that the AgNPs could damage the membrane integrity of Listeria monocytogenes cells and induce the release of nucleic acids from the cells, thereby disrupting cell reproduction.