生物炭
大肠杆菌
微生物学
噬菌体
噬菌体疗法
铜绿假单胞菌
生物
抗生素耐药性
细菌
泰特
假单胞菌
抗生素
化学
基因
生物化学
遗传学
有机化学
抑制因子
转录因子
热解
作者
Mao Ye,Mingming Sun,Yuting Zhao,Wentao Jiao,Bing Xia,Manqiang Liu,Yanfang Feng,Zhongyun Zhang,Dan Huang,Rong Huang,Jiguang Wan,Ruijun Du,Xin Jiang,Feng Hu
标识
DOI:10.1016/j.envpol.2018.04.070
摘要
High abundances of antibiotic-resistant pathogenic bacteria (ARPB) and antibiotic resistance genes (ARGs) in agricultural soil-plant systems have become serious threats to human health and environmental safety. Therefore, it is crucial to develop targeted technology to control existing antibiotic resistance (AR) contamination and potential dissemination in soil-plant systems. In this work, polyvalent bacteriophage (phage) therapy and biochar amendment were applied separately and in combination to stimulate ARPB/ARG dissipation in a soil-lettuce system. With combined application of biochar and polyvalent phage, the abundance of Escherichia coli K-12 (tetR) and Pseudomonas aeruginosa PAO1 (ampR + fosR) and their corresponding ARGs (tetM, tetQ, tetW, ampC, and fosA) significantly decreased in the soil after 63 days' incubation (p < 0.05). Similar results for endophytic K-12 and PAO1, and ARGs, were also obtained in lettuce tissues following combined treatment. Additionally, high throughput sequencing revealed that biochar and polyvalent phage synergetically improved the structural diversity and functional stability of the indigenous bacterial communities in soil and the endophytic ones in lettuce. Hence, this work proposes a novel biotechnology that combines biochar amendment and polyvalent phage therapy to achieve targeted inactivation of ARPB, which stimulates ARG dissipation in soil-lettuce systems.
科研通智能强力驱动
Strongly Powered by AbleSci AI