生物
传染性
病毒学
中和
流式细胞术
病毒定量
病毒
印版阅读器
荧光
高通量筛选
分子生物学
生物化学
量子力学
物理
作者
Yongpeng Sun,Wei Zhang,Qinjian Zhao,Jian Cao,Zhang Lujing,Jiang-Yan Xiang,Jun-Qiang Si,Xue Lin,Li Chen,Zizheng Zheng,Ningshao Xia
标识
DOI:10.1016/j.jviromet.2018.07.004
摘要
A licensed vaccine for respiratory syncytial virus (RSV) has yet to be developed, and a reliable and repeatable neutralizing assay is indispensable for vaccine development. Here, we demonstrated an optimized high-throughput RSV neutralization assay that utilizes a fluorescence plate reader (reader) as a substitute for flow cytometry to detect fluorescent signals in RSV-A2 mKate-infected cells. Furthermore, this study tested the influence of virus input and infectivity on the neutralizing assay and highlighted critical factors (together with a suggested protocol) for obtaining stable data using this assay.
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