Changes in physico-chemical properties, microstructure and intermolecular force of preserved egg yolk gels during pickling

酸洗 蛋黄 微观结构 分子间力 化学 蛋清 食品科学 结晶学 有机化学 物理化学 分子
作者
Yuan‐Han Yang,Yan Zhao,Mingsheng Xu,Na Wu,Yao Yao,Huaying Du,Haiyan Liu,Yonggang Tu
出处
期刊:Food Hydrocolloids [Elsevier BV]
卷期号:89: 131-142 被引量:71
标识
DOI:10.1016/j.foodhyd.2018.10.016
摘要

Changes in physical and chemical properties, microstructure and intermolecular interactions of preserved egg yolk gels during pickling were determined to clarify the formation mechanism of alkali-induced egg yolk gels. The pH, hardness, springiness, and cohesiveness of exterior egg yolks (hardened portion) increased firstly and then decreased, but moisture content decreased. The pH and viscosity of interior egg yolks (viscous portion) increased gradually, moisture content and oil exudation increased firstly and then decreased. Oil exudation of exterior egg yolks has similar trend with interior egg yolks. T21 and T22 of interior egg yolks obtained from LF-NMR spin-spin relaxation times decreased firstly and then increased. While T21 and T22 of exterior egg yolks showed an opposite trend and then increased at late state of pickling. Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) observed that interior egg yolk particles were ruptured and aggregated again, and exterior egg yolks gradually formed a dense and fiber gel network structure. Electrophoresis results showed that there was different degree degradation from interior to exterior egg yolks. Secondary structure contents contained numerous β-structure, regardless any portions of preserved egg yolks. However, exterior egg yolks had no thermal transformation, while interior egg yolks had significant thermal transformation, revealing the residual enthalpy decreased. The results suggested that the three-dimensional structure of exterior egg yolk gels was maintained by ionic bonds (71%), hydrophobic interaction (10%) and disulfide bond (10%), whereas the mainly intermolecular forces of interior egg yolk are ionic bond (80%) and the hydrogen bond (12%).
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