核酸
催化作用
信号(编程语言)
化学
生物物理学
组合化学
纳米技术
生物化学
材料科学
生物
计算机科学
程序设计语言
作者
Yu Jiang,Sanchita Bhadra,Bingling Li,Andrew D. Ellington
标识
DOI:10.1002/ange.201307418
摘要
Abstract Catalytic hairpin assembly (CHA) has previously proven useful as a transduction and amplification method for nucleic acid detection. However, the two hairpin substrates in a CHA circuit can potentially react non‐specifically even in the absence of a single‐stranded catalyst, and this non‐specific background degrades the signal‐to‐noise ratio. The introduction of mismatched base pairs that impede uncatalyzed strand exchange reactions led to a significant decrease of the background signal, while only partially damping the signal in the presence of a catalyst. Various types and lengths of mismatches were assayed by fluorimetry, and in many instances, our MismatCHA designs yielded 100‐fold increased signal‐to‐background ratios compared to a ratio of 4:1 with the perfectly matched substrates. These observations could be of general utility for the design of non‐enzymatic nucleic acid circuits.
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