Bee‐associated fungi mediate effects of fungicides on bumble bees

Bumble bees are important pollinators that face threats from multiple sources, including agrochemical application. Declining bumble bee populations have been linked to fungicide application, which could directly affect the fungi often found in the stored food and gastrointestinal (GI) tract of healthy bumble bees. We test the hypothesis that fungicides impact bee health by disrupting bee–fungi interactions. We examined the interactive effects of the fungicide propiconazole and fungal supplementation on the survival, reproduction and microbiome composition of microcolonies (queenless colonies) using two species, Bombus vosnesenskii and B. impatiens. We found that in B. vosnesenskii, fungicide exposure decreased survival, while fungal supplementation mitigated fungicide effects. For B. impatiens, fungicide application had no effect, but fungal supplementation improved survival and offspring production. Fungicides reduced fungal abundance in B. vosnesenskii microcolonies, but not in B. impatiens, where instead fungal addition decreased fungal abundance (ITS copy number). Fungal composition varied between treatments but differently between bee species. In B. impatiens, fungal addition increased microbiome diversity. In B. vosnesenskii, the abundance of the pathogen Ascosphaera was negatively associated with survival, while the yeast Zygosaccharomyces was positively associated with survival. Our results highlight that bumble bee species differ in response to fungicides and in the nature of bee-fungi associations. Fungicides can alter bee–fungi interactions with consequences for bee survival and reproduction, and exploring the mechanisms of such interactions, including interactions among fungi in the bee GI tract, may offer insights into bumble bee biology and conservation strategies.