Obstructive Apneas in a Mouse Model of Congenital Central Hypoventilation Syndrome

医学 先天性中枢性低通气综合征 阻塞性睡眠呼吸暂停 通气不足 呼吸暂停 脑干 四分位间距 麻醉 呼吸系统 内科学
作者
Amélia Madani,Gabriel Pitollat,Éléonore Sizun,Laura Cardoit,Maud Ringot,Thomas Bourgeois,Nélina Ramanantsoa,Christophe Delclaux,Stéphane Dauger,Marie‐Pia d’Ortho,Muriel Thoby‐Brisson,Jorge Gallego,Boris Matrot
出处
期刊:American Journal of Respiratory and Critical Care Medicine [American Thoracic Society]
卷期号:204 (10): 1200-1210 被引量:17
标识
DOI:10.1164/rccm.202104-0887oc
摘要

Rationale: Congenital central hypoventilation syndrome (CCHS) is characterized by life-threatening sleep hypoventilation and is caused by PHOX2B gene mutations, most frequently the PHOX2B27Ala/+ mutation, with patients requiring lifelong ventilatory support. It is unclear whether obstructive apneas are part of the syndrome. Objectives: To determine if Phox2b27Ala/+ mice, which present the main symptoms of CCHS and die within hours after birth, also express obstructive apneas, and to investigate potential underlying mechanisms. Methods: Apneas were classified as central, obstructive, or mixed by using a novel system combining pneumotachography and laser detection of abdominal movement immediately after birth. Several respiratory nuclei involved in airway patency were examined by immunohistochemistry and electrophysiology in brainstem-spinal cord preparations. Measurements and Main Results: The median (interquartile range) of obstructive apnea frequency was 2.3 (1.5-3.3)/min in Phox2b27Ala/+ pups versus 0.6 (0.4-1.0)/min in wild types (P < 0.0001). Obstructive apnea duration was 2.7 seconds (2.3-3.9) in Phox2b27Ala/+ pups versus 1.7 seconds (1.1-1.9) in wild types (P < 0.0001). Central and mixed apneas presented similar significant differences. In Phox2b27Ala/+ preparations, the hypoglossal nucleus had fewer (P < 0.05) and smaller (P < 0.01) neurons, compared with wild-type preparations. Importantly, coordination of phrenic and hypoglossal motor activities was disrupted, as evidenced by the longer and variable delay of hypoglossal activity with respect to phrenic activity onset (P < 0.001). Conclusions: The Phox2b27Ala/+ mutation predisposed pups not only to hypoventilation and central apneas, but also to obstructive and mixed apneas, likely because of hypoglossal dysgenesis. These results thus demand attention toward obstructive events in infants with CCHS.
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