Current trends of food habits to lead a modern lifestyle with inadequate sleep have become one vital aspect to make our gut unhealthy. Infective gut with inflamed ulcer has now become an overgrowing concern to the whole world. The human gut coevolved with several microorganisms that maintain a symbiotic relationship and influence the gut to sustain a proper shape and function. Thus, dysbiosis or misbalancing in gut microbiome leads to various health issues that not only are restricted to the gut such as inflammatory bowel disease, cancer, diarrhea, and allergy but also affect the heart, kidney, and liver. Therefore, to overcome such life-threatening outcomes, a therapeutic solution is needed to select and have a sufficient potential in rebuilding eubiosis in the gut. In this concerned period, probiotics can be a promising prophylaxis as several studies explored its capabilities as life savior from such diseases. Herein, we emphasize the role of probiotics in treating gut-related inflammatory and infectious diseases on a molecular and cellular basis.